Polysorbate and Solubilizer

　3) Hemolysis Test

Samples containing a certain concentration of conventional Polysorbate80, a competitor's Polysorbate80, and our highly pure Polysorbate80, each of an injection grade, were added to guinea pig erythrocytes and their hemolyzing properties were evaluated after 60 minutes.
The highly pure Polysorbate80 showed a much lower tendency to produce hemolysis, despite the fact that all three samples contained surfactants with substantially the same molecular structure.
We are convinced that this provides evidence that our Polysorbate80 has a low cytotoxicity and is a highly safe product if used correctly.
Moreover, the highly pure Polysorbate80 has an excellent oxidative stability. This means that our high-purity Polysorbate80 is worthy of being adopted as a standard worldwide, in that it has the high stability that is required in both the design and production of formulations, and the high level of safety required for use as a component of pharmaceutical products.

Effect of Concentration of Polysorbate 80 on Hemolytic Ratio. Red Blood cells from guinea pig are treated with Polysorbate 80 for 60 mins. Each hemolytic ratio is determined by absorbance of 576nm in the solution.